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The special edition is devoted to issues related to applying Positron Annihilation Spectroscopy as a nondestructive technique for studying voids and defects in solids. The first chapter contains articles that analyse the features of the latest techniques and instrumentation and present the results of developing some of them. The second chapter is devoted to the practical application of the methods of positron annihilation spectroscopy for studying and analysing defects in alloys, multilayer graphene, photoconductor thin films, complex iron-containing oxide glasses, for the early detection of mental disorders and cancer, etc. Without a doubt, this special edition will be useful for both beginners and professionals with experience in the field of positron annihilation spectroscopy.