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In the last three decades, the fast development of single-molecule techniques has revolutionized the way we observe and understand biological processes. Some of these techniques have been further adapted as tools for bioanalysis. This book summarizes and details the frontiers of the development of these tools as well as their applications. The contributors are young and established researchers in their respective fields. The main content originates from the lecture notes of a chemistry graduate course taught by the book editor at Nanjing University. This book is suitable to be used as a textbook for a high-level undergraduate or an entry-level graduate course. The systematically written content provides a thorough illustration of the mechanisms of each methodology presented.
Shuo Huang obtained his BS in physics from Nanjing University, China in 2006 and his PhD in biophysics from Arizona State University in 2011. From 2011 to 2015, he was a postdoctoral fellow at the University of Oxford. In 2015, Prof. Huang joined Nanjing University, where he is Professor at the School of Chemistry and Chemical Engineering, State Key Laboratory of Analytical Chemistry for Life Sciences, Chemistry and Biomedicine Innovation Center (ChemBIC). His research focuses on the development of single-molecule sensing tools based on biological nanopores.