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This second edition volume expands on the previous edition with new chapters discussing the latest protocols that encompass the detection, characterization, and genomic analysis of Cryptosporidium. The first few chapters in this book focus on diagnostic assays, such as traditional microscopy-based techniques, nucleic acid-based molecular detection, and immunoassay-based methodologies. Several chapters cover whole genome sequencing methodologies, genotyping approaches, and novel techniques such as hybridization bait capture analyses. A significant portion of this book is dedicated to functional genomics and experimental models, with discussions on tools used for genetic manipulation (including CRISPR/cas9), gene silencing, and host-pathogen interactions. The volume concludes with chapters exploring drug discovery and testing, highlighting high-content screening methods, phenotypic assays, and efficacy testing of potential disinfectants and therapeutics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Cryptosporidium: Methods and Protocols, Second Edition is a valuable tool for researchers interested in gaining more insight on foundational and innovative Cryptosporidium research, which will inspire future advancements in this field.