Rapid Virus Diagnosis

Rapid Virus Diagnosis: Application of Immunofluorescence presents developments in immunofluorescence as a technique for the diagnosis of virus infections. It discusses the effective and rapid methods for the diagnosis of virus infections. It addresses the application of fluorescent antibody techniques to the diagnosis and the investigation of virus infections and the assessment of their value both to the clinician and to the virologist. Some of the topics covered in the book are the fluorescence microscopy; nature of fluorescence; filter systems; transmitted light microscopy; interference filters for transmitted light microscopy; lamp centration and alignment of microscope; contrast-fluorescence condensers; photomicrography; and choice of microscope. The culture of viruses for production of antiserum is fully covered. The selection of cell lines or tissues for virus culture is discussed in detail. The text describes in depth the fluorescent antibody staining techniques. The preparation of specimens from respiratory infections is presented completely. A chapter is devoted to the respiratory syncytial virus. Another section focuses on the analysis influenza virus, paramyxoviruses, and picornaviruses. The book can provide useful information to doctors, virologists, students, and researchers.

Contents1. Introduction2. Fluorescence MicroscopyNature of FluorescenceLight SourcesFilter SystemsViewing HeadsObjectivesOcularsPhotomicrographyChoice of Microscope3. The ReagentsCulture of Viruses For Production of AntiserumProduction of AntiserumConjugates4. Immunofluorescence Techniques, Control of Specificity and Nonspecific FluorescenceFluorescent Antibody Staining TechniquesControl of Specificity of Fluorescent Antibody Staining Techniques MountantsNon-Specific FluorescenceAbsorption Techniques5. Preparation of SpecimensPreparation of Specimens From Respiratory InfectionsPreparation of Specimens From Infections Not Involving Respiratory TractPreparation of Tissue Culture Cells For Fluorescent Antibody Investigations6. Respiratory Syncytial Virus7. Influenza Viruses8. ParamyxovirusesParainfluenza VirusesMumps Virus9. Measles (Morbilli)10. Picornaviruses11. RabiesTechnical Considerations12. Variola13. AdenovirusesImmunofluorescence and Tissue CultureDirect Examination of Specimen by Immunoflourescence For Diagnosis of Adenovirus InfectionThe Diagnosis of Adenovirus Infections of the Eye14. Arboviruses (Including Togaviruses, Arenaviruses, Colorado Tick Fever)15. Rubella16. Herpesvirus HominisInvestigation of Skin Lesions by ImmunofluorescenceInvestigation of Infections of the Conjunctivae For HVH by ImmunofluorescenceUse of Immunofluorescence For Identifying HVH in Tissue CultureImmunofluorescence and Antiviral Treatment of HVH Eye InfectionsHVH and EncephalitisDisseminated HVH Infection of the NewbornTypes of HVH17. Miscellaneous Viruses in the Herpesvirus GroupHerpes Varicellae-Herpes ZosterCytomegalovirus of ManEpstein-Barr Virus18. Detection of Virus-Specific IgM by ImmunofluorescenceMethods For Demonstration of Virus-Specific IgM ActivitySources of ErrorCytomegalovirusRubellaHerpesvirus HominisEpstein-Barr VirusMumpsInfluenza ARotavirusMeasles19. Application of Immunofluorescence Technique To Clinical Virology Management of Patients and Rational Use of Antibiotics The Use of Antiviral Drugs Virus Infection Outside Acute Stage of Illness The Use of Rapid Diagnostic Techniques in Fatal Infections Virus Infection in the Immunosuppressed Patient AndIts Management Frequently Occurring Viruses Producing Unusual Symptoms Viruses Difficult To Cultivate in the Laboratory Prevention of Ward Cross-Infection With Viruses Virus Diagnosis in Areas Distant From A Virus Laboratory Rapid Diagnosis in Epidemiology Immunofluorescence As A Laboratory Aid Index