Standard Methods of Clinical Chemistry

Standard Methods of Clinical Chemistry, Volume 6 provides information pertinent to the more accurate methods of analysis. This book deals with various subjects, including personnel management, electronics, and data processing systems. Organized into 21 chapters, this volume begins with an overview of the most colorimetric methods for estimating uric acid based on the nonspecific reduction of phosphotungstate by uric acid in an alkaline solution. This text then examines the electrophoretic separation and quantitation of proteins in serum or other body fluids. Other chapters provide a discussion of the control of the major reaction variables needed to meet the recommendations of the International Union of Biochemistry. This book discusses as well the modifications developed to eliminate some of the inaccuracies resulting from incomplete destruction of hydrogen peroxide and instability of the developed chromophore. The final chapter deals with the enzymatic methods for the determination of lactic and pyruvic acids in body fluids and tissues. This book is a valuable resource for clinical chemists.

Harry H. Sobotka-A Biographical SketchContributorsPrefaceUric Acid (Uricase)Cellulose Acetate Electrophoresis of Protiens of Serum, Cerebrospinal Fluid, and UrineThe International Unit: Standardization of Enzyme Activity UnitsHydroxyproline in Urine and TissuesPorphyrins, Porphobilinogen, and Aminolevulinic Acid in UrineToxicology in a General HospitalSpectrophotometric Quantitation of Plasma and Urinary Amino Nitrogen with FluorodinitrobenzeneColorimetric Determination of Vanilmandelic Acid in UrineHemoglobin in PlasmaIon-Exchange Method for the Determination of Plasma Ammonia Nitrogen with the Berthelot ReactionElectrophoretic Determination of Serum Lipoproteins (Prestaining Technique)The Determination of Total Seromucoid (as Hexose)Glatamic Oxalacetic Transaminase (Colorimetric)The Determination of Glocuse by the Ortho-Toluidine Method (Filtrate and Direct Procedure)Oxygen, Partial Pressure (PO2) Electrode MethodIodometric Measurement of Amylase (Caraway)Serum Lithium Determination by Atomic Absorption SpectroscopyCalculiCalcium (Fluorometric)Triglycerides ("Neutral Fat") in SerumNomenclature for Quantities and UnitsEnzymatic Methods for Lactic and Pyruvic AcidsAuthor Index 261Subject Index