Analytical Applications of Immobilized Enzymes and Cells

Analytical Applications of Immobilized Enzymes and Cells, Volume 3 presents a survey of the interdisciplinary interactions, developments, and trends on research in immobilized enzymes and cells. This volume is comprised of eight chapters. Chapters 1 to 4 are devoted to the description of methodologies and instrumentations which are used in routine analysis. Among those described are enzyme tubes, enzyme thermistors, and enzymic or microbial electrodes. Chapter 4 provides a theoretical analysis of electrode design. Subsequent chapters focus on solid-phase enzyme immunoassays and on techniques for in vivo monitoring of metabolites; economic evaluation of the use of high-purity enzymes in analysis; and an assessment of the significance of microprocessors and computer science in enzyme based analysis. Chemists and chemical engineers will find the book very useful.

List of ContributorsPrefaceContents of Previous VolumesOpen Tubular Heterogeneous Enzyme Reactors in Continuous-Flow Analysis I. Introduction II. Continuous-Flow Analysis III. Enzymes in Continuous-Flow Analysis IV. Heterogeneous Enzyme Kinetics V. Theory of Open Tubular Heterogeneous Enzyme Reactors (OTHERs) with Segmented Flow VI. Preparation and Properties of OTHERs VII. Factors Affecting Performance of OTHERs VIII. Other Uses of OTHERs IX. Conclusions Appendix I: Procedures for the Preparation of Chemically Modified Nylon Tubes Appendix II: Examples of Analytical Applications of OTHERs List of Symbols ReferencesEnzyme Thermistor Devices and Their Analytical Applications I. Introduction II. Experimental III. Applications IV. Comments on the Technique V. Concluding Remarks ReferencesBioelectrochemical Sensors Based on Immobilized Enzymes, Whole Cells, and Proteins I. Introduction II. Bioelectrochemical Sensors Based on Immobilized Enzymes III. Bioelectrochemical Sensors Based on Immobilized Whole Cells IV. Bioelectrochemical Sensors Based on Immobilized Proteins ReferencesTheoretical Aspects of Enzyme Electrode Design I. Introduction II. Background III. Classification and Physical Description IV. Formulation of the Models V. Conclusions Nomenclature ReferencesSolid Phase Enzyme Immunoassays I. Introduction II. Quantitation Using Solid Phase Enzyme Immunoassays III. Reagents Used In Solid Phase Enzyme Immunoassays IV. Concluding Comments Appendix: Enzyme-immunoassay Procedures ReferencesIn Vivo Monitoring of Metabolites Using Enzyme-Based Analytical Techniques I. Introduction II. Development of an in Vivo Monitoring System III. Future Development IV. Conclusions ReferencesEconomic Aspects of High-Purity Enzymes as Related to Analysis I. Introduction II. The Scope of Enzymatic Analysis III. Costs of Enzyme Purification IV. The Cost Structure of Organization in the Clinical Laboratory V. The Free Soluble and Immobilized Enzyme from the Standpoint of Methodology VI. Conclusions ReferencesPotential Impacts of Physics and Electronics on Enzyme-Based Analysis I. Introduction II. General Aspects of Transducers III. Microprocessors IV. Microfabrication Technology V. Examples of Classes of Transducers of Relevance VI. Conclusion ReferencesIndex