Applied Electrospray Mass Spectrometry

Discussing strategies to determine the structure and machanisms of numerous compound classics, this book covers new chemical and elctrophoretic techniques for rapid sample preconcentration and separation. It summarizes breakthroughs in the theory and instrumentation of electrospray mass spectrometry in pharmaceutical and biomedical applications, provides practical examples for the characterization of peptides, proteins, and glycoproteins, includes applications in proteomics, combinatorial chemistry, and drug characterization. Topics include chemical and electrophoretic techniques for rapid sample preconcentration and separation, screening processes for proteins from libraries of compounds, protein folding and dynamics, and more.

Birendra N. Pramanik (Edited by) , A. K. Ganguly (Edited by) , Michael L. Gross (Edited by)

Preface iii Contributors vii 1 Electrospray Ionization Mass Spectrometry: History, Theory, and Instrumentation Robert B. Cody 1 2 Nanospray Electrospray Ionization Development: LC/MS, CE/MS Application Tom R. Covey and Devanand Pinto 105 3 Characterization of Pharmaceuticals and Natural Products by Electrospray Ionization Mass Spectrometry A. K. Ganguly; Birendra N. Pramanik, Guodong Chen, anti Petia A. Shipkova 149 4 Electrospray Mass Spectrometry Applications in Combinatorial Chemistry 187 Mike S. Lee 5 Electrospray Mass Spectrometry in Contemporary Drug Metabolism and Pharmacokinetics 211 Mark J. Cole, John S. Janiszewski, and Hassan G. Fouda 6 Electrospray Mass Spectrometry of Peptides and Proteins: Methodologies and Applications 251 Joseph A. Loo and Greg W. Kilby 1 Structural Analysis of Glycoproteins by Electrospray Ionization Mass Spectrometry 283 Anthony Tsarbopoulos, Ute Bahr, Birendra N. Pramanik, and Michael Karas 8 Advanced Mass Spectrometric Approaches for Rapid and Quantitative Proteomics 307 Richard D. Smith, Gordon A. Anderson, Thomas P. Conrads,Christophe D. Masselon, Mary S. Lipton, Ljiljana Pasa Tolic, and Timothy D. Veenstra 9 Detection of Noncovalent Complexes by Electrospray Ionization Mass Spectrometry 361 A. K Ganguly, Birendra N. Pramanik, Guodong Chen, and Anthony Tsarbopoulos 10 Application of Hydrogen Exchange and Electrospray Ionization Mass Spectrometry in Studies of Protein Structure and Dynamics 389 Yinsheng Wang and Michael L. Gross 11 Microelectrospray Analysis Combined with Microdialysis Sampling of Neuropeptides and Drugs 411 Per E. Andren and Richard M. Caprioli Index 431