Chemical and Biochemical Applications of Lasers V4

Chemical and Biochemical Applications of Lasers, Volume IV focuses on the practical applications of standard commercial laser systems. This book examines the structural studies of DNA by fluorescence microscopy and discusses photochemistry and structural spectroscopy. Organized into eight chapters, this volume starts with an overview of a few cases of laser-induced fluorescence studies of biological molecules. This text then examines the sharp fluorescence spectra of complex molecules in solids that are obtained when a narrow-band laser selectively excites molecules in particular sites. Other chapters describe the theory and application of resonance Raman spectroscopy to various biological systems. This book provides as well a thorough treatment of coherent anti-Stokes Raman spectroscopy and its application in combustion diagnostics and analytical chemistry. The final chapter explores laser control of the sequential photochemical reaction of the drug psoralen with the two strands of the DNA double helix. Physicists, chemists, electrochemists, and chemical engineers will find this book useful.

List of ContributorsPreface1. Structural Studies of Biological Molecules via Laser-Induced Fluorescence: Acridine-DNA Complexes I. Introduction II. The Laser Microfluorometer III. The Acridine-DNA Complex IV. Laser-Induced Fluorescence Experiments on Acridine-DNA Complexes V. Conclusions and Prospects References2. Site Selection Spectroscopy I. Introduction II. Optical Spectra in Condensed Phases III. Site Selection Spectroscopy IV. Conclusions References3. Biological Applications of Resonance Raman Spectroscopy in the Visible and Ultraviolet: Visual Pigments, Purple Membrane, and Nucleic Acids I. Introduction II. Theory III. Visual Pigments IV. Purple Membrane Protein V. Ultraviolet Resonance Raman VI. Multichannel Detectors in Raman Spectroscopy VII. Conclusion References4. Laser Excitation of Raman Scattering from Adsorbed Molecules on Electrode Surfaces I. Introduction II. Sensitivity Requirements for the Implementation of Surface Raman Spectroscopy III. The Surface Enhanced Raman Effect IV. Survey of Experimental Results and Discussion V. Prospects for the Future of SRS VI. References5. Coherent Anti-Stokes Raman Spectroscopy I. Introduction II. Theory III. Practical Considerations IV. Experimental Results V. Conclusion References6. Theory of Molecular Rate Processes in the Presence of Intense Laser Radiation I. Introduction II. Theoretical Methods III. Applications References7. Multiphoton Dissociation of Gas Phase Ions Using Low Intensity cw Laser Radiation I. Introduction II. Ion Cyclotron Resonance Spectroscopy III. Studies of Multiphoton Dissociation Processes Using Visible Radiation IV. Multiphoton Dissociation of Ions with Low Intensity cw Infrared Laser Radiation V. Mechanism of Infrared Multiphoton Dissociation VI. Summary and Prognosis References8. Photochemical Fixation of the Nucleic Acid Double Helix Utilizing Psoralens I. Introduction II. Rapid Pulse Kinetics of Psoralen Photoaddition to DNA and of Psoralen Cross-Link Formation III. Utilization of High Laser Intensities in a Kinetic Analysis of Psoralen Intercalation in the DNA Helix IV. Conclusion ReferencesIndex