From Gene to Protein: Translation into Biotechnology

From Gene to Protein: Translation into Biotechnology is the 15th volume in the continuing series under the title ""Miami Winter Symposia"". The theme of the symposium is the translation of the basic research findings into the practical application of biotechnology. This book summarizes methodology and its applications that lie behind the practical innovations. The book starts with reviews of techniques of eukaryotic cell culture, hybridoma technology and uses, and the in vitro synthesis of DNA and its use in the generation of protein analogs. Considerable space is devoted to development of monoclonal antibodies that promises to be the dominating tool of medical technology, both for diagnosis and therapy. Cloning into eukaryotic cells and methods of increasing the levels of gene expression are included. These topics reflect areas of intensive research that have important commercial and clinical value. Core chapters describe biological activities of cloned gene products, including reports on trials with human subjects of interferon, human insulin, and growth hormone. A panel session on horizons in biotechnology is also provided, looking forward to the directions of future research and its applications. Biotechnologists, cell biologists, scientists, researchers, teachers, and students will greatly benefit from this book.

Speakers and DiscussantsPrefaceThe Thirteenth Lynen Lecture Messing About with Isotopes and Enzymes and AntibodiesIntroduction Moving Genes: Promises Kept and PendingDISCUSSION: D. C. Burke, G. Haughton, G. F. Merrill, and C. SimonsenTechniques of Eukaryotic Cell Culture Cell Culture and PhysiologyGordon H. SatoDISCUSSION: D. Balinsky, D. T. Denhardt, and G. Knapp Regulation of Growth and Differentiation of Mammalian Cells by Hormones and Extracellular MatrixDISCUSSION: H. Busch and R. G. Smith Microcarriers and the Problem of High Density Cell CultureDISCUSSION: D. H. Gelfand and G. H. Sato Crown Gall-Nature's Genetic EngineerDISCUSSION: J. R. Geiger and K. SirotkinMonoclonal Antibodies-Production and Uses Monoclonal Antibodies: The Production of Tailor-Made Serological ReagentsDISCUSSION: A. A. Ansari, N. L. Levy, P. A. Liberti, and W. I. Schaeffer Monoclonal Antibodies in the Analysis of the Molecular Basis for Human Genetic DiseasesDISCUSSION: J. Harford, R. J. Hay, J. L. Strominger, and R. L. Ward Human Cell Surface Antigens Studied with Monoclonal AntibodiesDISCUSSION: D. H. Gelfand Immunocytochemistry with Monoclonal Antibodies: Potential Applications in Basic Sciences and Histopathology Human Immunoglobulin Expression in Hybrid CellsDISCUSSION: S. I. Chavin, J. W. Larrick, and J. L. StromingIn Vitro Synthesis of DNA and the Generation of Protein Analogs The Role of Synthetic DNA in the Preparation of Structural Genes Coding for Proteins DISCUSSION: A. A. Ansari and U. R. Muller The Synthesis and Biochemical Reactivity of Biologically Important Genes and Gene Control Regions DISCUSSION: H. Heyneker, and unknown speaker Synthesis of Human Interferons and Analogs in Heterologous Cells DISCUSSION: M. Bina, D. Botstein, D. C. Burke, V. Chowdhry, M. Chretien, C. Colby, D. V. Hendrick, P. A. Liberti, U. R. Muller, S. A. Sherwin, S. L. C. Woo, H. Young, and J. B. ZeldisCloning into Eukaryotic Cells Making Mutations in Vitro and Putting Them Back into Yeast DISCUSSION: D. Gelfand, D. Schlessinger, K. Sirotkin, and A. M. Skalka Expression of the Human Growth Hormone Gene Is Regulated in Mouse Cells DISCUSSION: R. Meagher, M. Nilsen-Hamilton, and M. Pater Transfer of the Mouse Metallothionein-I Gene into Cultured Cells and into Animals DISCUSSION: M. Bina, D. Botstein, H. Faber, and J. MillsIncreasing Levels of Gene Expression Construction of Three Hybrid Promoters and Their Properties in Escherichia coli DISCUSSION: D. Botstein and D. GelfandBiological Activities of Cloned Gene Products Regulation of Histone Gene Expression in Human Cells DISCUSSION: M. Adesnik, B. Nelkin, and P. Reczek Clinical Investigation of Partially Pure and Recombinant DNA-Derived Leukocyte Interferon in Human Cancer DISCUSSION: D. Burke, J. Cahill, V. Chowdhry, P. A. Libera, and H. Young The Use of Biosynthetic Human Insulin in Man DISCUSSION: S. King The Biological Effectiveness of Pituitary-Derived and Biosynthetic Methionyl-hGH in Animals and Man Biological Activity of a Cloned Human Enzyme Urokinase DISCUSSION: H. Heyneker and unknown speaker Activity of Cloned Gene Products in Animal Systems DISCUSSION: L. Baumbach, D. Burke, D. Gelfand, and R. Ward Studies on Poliovirus Produced by Transfection with Cloned Viral cDNA DISCUSSION: D. Burke, E. Lamon, R. Samulski, and N. StebbingHorizons in Biotechnology Friday Afternoon Panel Discussion Free Communications Genes for Cytochrome P-450 and Their Regulation Localization of a Heparin Binding Site of Fibronectin with the Aid of Monoclonal Antibodies Nucleotide Sequence on the gig C Gene Coding for ADP-Glucose Synthetase from E.