Bioconjugate Techniques

Bioconjugate Techniques is the essential guide to the modification and crosslinking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or crosslinking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers. Armed with this information and the abundant protocols provided, readers will form unique complexes that can be used for detecting, quantifying, and targeting important analytes. This book helps readers make: high activity antibody-enzymes conjugates, immunotoxins, immunogen complexes, liposome conjugates; as well as biotinylated molecules, avidin or streptavidin conjugates, colloidal gold labeled proteins, PEG or dextran complexes, labeled oligonucleotide probes, and fluorescently tagged or radiolabeled molecules.This book is the first to thoroughly capture the entire field of bioconjugate chemistry in a single volumeServes as a practical guide to modification and cross-linking technology for research, diagnostics, and therapeuticsProvides useful, detailed, easy-to-follow, step-by-step protocolsContains easy-to-read, and easy-to-understand key concepts for making bioconjugates of all typesEfficiently covers the chemistry of bioconjugation, the major reagents available for modification and cross-linking, and the application of these reagents to the synthesis of highly active conjugatesCites over more than references keyed to concepts covered in the bookUses more than 600 figures to illustrate bioconjugate reagents, their reactions, and applicationsSuggests sources for all key reagents

Bioconjugate Chemistry: Functional Targets. The Chemistry of Reactive Groups. Bioconjugate Reagents: Zero-Length Cross-linkers. Homobifunctional Cross-linkers. Heterobifunctional Cross-linkers. Trifunctional Cross-linkers. Cleavable Reagent Systems. Tags and Probes. Bioconjugate Applications: Preparation of Hapten-Carrier Immunogen Conjugates. Antibody Modification and Conjugation. Immunotoxin Conjugation Techniques. Preparation of Liposome Conjugates and Derivatives. Avidin-Biotin Systems. Preparation of Colloidal-Gold-Labeled Proteins. Modification with Synthetic Polymers. Enzyme Modification and Conjugation. Nucleic Acid and Oligonucleotide Modification and Conjugation. Subject Index.