Bioconjugate Techniques

Bioconjugate Techniques, 2nd Edition, is the essential guide to the modification and cross linking of biomolecules for use in research, diagnostics, and therapeutics. It provides highly detailed information on the chemistry, reagent systems, and practical applications for creating labeled or conjugate molecules. It also describes dozens of reactions with details on hundreds of commercially available reagents and the use of these reagents for modifying or cross linking peptides and proteins, sugars and polysaccharides, nucleic acids and oligonucleotides, lipids, and synthetic polymers.- A one-stop source for proven methods and protocols for synthesizing bioconjugates in the lab- Step-by-step presentation makes the book an ideal source for researchers who are less familiar with the synthesis of bioconjugates- More than 600 figures that visually describe the complex reactions associated with the synthesis of bioconjugates- Includes entirely new chapters on the latest areas in the field of bioconjugation as follows: Microparticles and nanoparticlesSilane coupling agentsDendrimers and dendronsChemoselective ligationQuantum dotsLanthanide chelatesCyanine dyesDiscrete PEG compoundsBuckyballs,fullerenes, and carbon nanotubesMass tags and isotope tagsBioconjugation in the study of protein interactions

PART I Bioconjugate Chemistry1. Functional Targets2. The Chemistry of Reactive GroupsPART II Bioconjugate Reagents3. Zero-Length Cross-Linkers4. Homobifunctional Cross-linkers5. Heterobifunctional Cross-linkers6. Trifunctional Cross-linkers7. Dendrimers and Dendrons8. Cleavable Reagent Systems9. Fluorescent Probes10. Bifunctional Chelating Agents and Radioimmunoconjugates11. Biotinylation Reagents12. Iodination Reagents13. Silane Coupling Agents14. Microparticles and Nanoparticles15. Buckyballs, Fullerenes, and Carbon Nanotubes16. Mass Tags and Isotope Tags17. Chemoselective Ligation; Bioorthogonal Reagents18. Discrete PEG CompoundsPART III Bioconjugate Applications19. Preparation of Hapten - Carrier Immunogen Conjugates20. Antibody Modification and Conjugation21. Immunotoxin Conjugation Techniques22. Preparation of Liposome Conjugates and Derivatives23. Avidin - Biotin Systems24. Preparation of Colloidal-Gold-Labeled Proteins25. Modification with Synthetic Polymers26. Enzyme Modification and Conjugation27. Nucleic Acid and Oligonucleotide Modification and Conjugation28. Bioconjugation for the Study of Protein Interactions