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This book presents an authoritative collection of HLA phenotyping and genotyping techniques to be used at the bench level and as a reference. It includes detailed methodologies, notes on the interpretation of tests, reference material, and appendices.
Kam M. Hui (Nat'l University/Singapore) (Author) , Jeffrey L. Bidwell (University of Bristol, United Kingdom) (Author)
Introduction 1. PCR-RFLP 2. HLA-DR and -DQ Typing by DNA-RFLP Analysis 3. Rapid HLA-DR-Dw and DP Matching by PCR Fingerprinting and Related DNA Heteroduplex Technologies 4. A Comprehensive HLA-DRB, -DQB, and -DPB Oligotyping Procedure by Hybridization with Sequence-Specific Oligonucleotide Probes 5. HLA-DR Typing by Polymerase Chain Reaction Amplification with Sequence-Specific Primers (PCR-SSP) 6. Serological Typing of HLA-A, -B, and -C Antigens 7. HLA-DR and -DQ Serotyping 8. Definition of HLA-Dw Determinants Using Homozygous Typing Cells and the Mixed Lymphocyte Culture 9. Definition of HLA-Dw and HLA-DPw Determinants by the Primed Lymphocyte Test