Bacterial Immunoglobulin-Binding Proteins

Bacterial Immunoglobulin-Binding Proteins: Applications in Immunotechnology, Volume 2 covers the state of knowledge of bacterial immunoglobulin-binding proteins. The book focuses on practical approaches to isolation, characterization, and use of bacterial immunoglobulin-binding proteins. The majority of these studies involve the type I Fc-binding protein (staphylococcal protein A) and the type III Fc-binding protein (streptococcal protein G). Physiological chemists, pediatricians, and microbiologists will find the book invaluable.

ContributorsPreface 1 Introduction to bacterial immunoglobulin-binding proteins I. Introduction II. The Distribution and Functional Reactivity of Bacterial IgG Fc-Binding Proteins III. Bacterial IgG-Binding Proteins IV. Second Generation Immunoglobulin-Binding Proteins V. Summary References2 Detection and enhancement of expression of bacterial cell surface immunoglobulin-binding proteins I. Introduction II. Preparation of Bacteria III. Standardization of Bacteria IV. Assays for the Detection of Immunoglobulin-Binding Proteins V. Direct Binding of Immunoglobulins to Bacteria VI. Direct Binding Assay VII. Absorption of IgG by Bacteria VIII. Dot Blot Procedure IX. Methods to Enhance Immunoglobulin-Binding Proteins X. Mouse Passage XI. Colony Blot Selection XII. Storage of Strains XIII. Conclusions References3 Extraction and monitoring of soluble immunoglobulin-binding proteins I. Introduction II. Extraction Procedures III. Secreted IgG-Binding Proteins IV. Summary References 4 Isolation and functional characterization of bacterial immunoglobulin-binding proteins I. Introduction II. Selection of Immunoglobulin Source to Prepare Affinity Columns III. Nature of the Sample to Be Purified IV. Selection of Eluting Agent V. Affinity Purification of a Type III Fc-Binding Protein Solubilized by Bacteriophage Lysis of the Group C Streptococcus 26RP66 VI. Characterization of Affinity-Purified Immunoglobulin-Binding Proteins VII. Comparison of Functional Activities of Fc-Binding Proteins VIII. Summary References 5 Determination of protein-binding affinities among bacterial cell surface proteins I. Introduction II. Binding of Mammalian Proteins to Bacterial Surfaces: Screening Procedures III. Solubilization of Immunoglobulin-Binding Bacterial Surface Proteins IV. Determination of Immunoglobulin-Binding Protein in Low Concentrations V. Analysis of the Binding Immunoglobulins and Other Host Proteins to Purified, Bacterial Surface Proteins VI. Determination of the Affinity Constant for Binding between a Bacterial Cell Wall Protein and Its Ligand References 6. Production of polyclonal antibodies to immunoglobulin-binding proteins I. Introduction II. Selection of the Species of Animal to Immunize III. Preparation of Immunogen and Immunization IV. Kinetics of Antibody Production V. Detection of Antibody to Immunoglobulin-Binding Proteins VI. Conclusion References 7. Use of radiolabeled bacterial Fc-binding proteins as tracers for soluble antigens I. Introduction II. Competitive Inhibition Radioimmunoassay III. Summary References 8. Application of enzyme-labeled IgG-binding proteins in immunoassay I. Introduction II. Types of Assays III. Preparation of Immunoglobulin Fc-Binding Protein-Enzyme Conjugate Tracers IV. Development of an ELISA to Quantify Human IgA Using a Type III Fc-Binding Protein-Alkaline Phosphatase Conjugate as Tracer V. Summary References 9. Use of Fc-binding proteins to identify cell surface and secreted antigens associated with group B streptococci I. Introduction II. Group B Streptococcal Typing Nomenclature III. Two-Stage Radioimmunoassay for Detection of Group B Streptococcal Type-Specific Antigens IV. Adaptation of the Two-Stage RIA to a Dot Blot Assay V. Adaptation of the Two-Stage RIA to an ELISA Typing Procedure VI. Summary References 10. Application of Fc-binding proteins for the detection of specific antibodies I. Introduction II. Development of an Assay for Antibodies to a Soluble Antigen III. Detection of Antibodies to Tumor-Associated Antigens IV. Detection of Rabbit IgM Antibodies to Sheep Erythrocytes V. Summary References 11.