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This text details contemporary electroanalytical strategies of biomolecules and electrical phenomena in biological systems. It presents significant developments in sequence-specific DNA detection for more efficient and cost-effective medical diagnosis of genetic and infectious diseases and microbial and viral pathogens. The authors discuss the latest advances in amperometric biosensing, capillary electrophoresis, DNA amplification and detection, single-cell neurochemistry, in vivo electrochemistry, and electrochemical immunoassay. They also present recent techniques to evaluate the reaction properties of complex electron-transport enzymes and describe the use of fast-scan cyclic voltammetry (FSCV) to study the mechanisms and kinetics of dopamine neurotransmission, among other topics.
Anna Brajter-toth, James Chambers
Part I: Electrochemistry of DNA 1. Charge Migration Through the DNA Double Helix 2. Electrochemical DNA Biosensors 3. Amplified and Specific Electronic Transduction of DNA Sensing Processes in Monolayer and Thin-Film Assemblies Part II: Protein Electrochemistry 4. Direct Electrochemistry of Proteins and Enzymes at Electrodes 5. Voltammetric Investigations of Iron-Sulfur Clusters in Proteins 6. Polyion and Surfactant Films on Electrodes for Protein Electrochemistry 7. Electrochemistry of Peroxidases Part III: In Vivo Electrochemistry 8. Mechanisms and Kinetics of Neurotransmission Measured in Brain Slices with Cyclic Voltammetry 9. Electrochemical Monitoring of Exocytosis from Individual PC12 Cells in Culture Part IV: Bioelectroanalysis 10. Milestones of Electrochemical Immunoassay at Cincinnati 11. Electrochemical Detection of Peptides 12. Microfabrication of Electrode Surfaces for Biosensors 13. Electrocatalytic Determination of Biochemical Compounds Part V: Biological Applications 14. Electrodes Based on the Electrical "Wiring" of Enzymes 15. Capillary Electrophoresis/Electrochemistry: Instrument Design and Bioanalytical Applications 16. Ultrahigh Sensitivity Analysis of Amino Acids and Peptides by Capillary Liquid Chromatography with Electrochemical Detection
