Human Lymphokines

Human Lymphokines: The Biological Immune Response Modifiers is a collection of papers presented at the Third International Symposium on Human Lymphokines held on October 15-17, 1981 at the Wadley Institutes of Molecular Medicine in Dallas, Texas. Contributors explore the role of human lymphokines in immune responses and their applications in immunotherapy. The focus is on lymphokines that have either entered clinical trials or are nearing clinical evaluation. Some of the activities of possible immediate significance, such as Glia cell stimulating factor and polyclonal B-cell activator, are also described. This volume is organized into six sections encompassing 56 chapters and begins with an overview of assay systems, touching on topics such as the activation of macrophages by interferon to produce plasminogen and macrophage activation by lymphokine factors. The next sections turn to the production, purification, and biological and chemical characterization of lymphokines. The discussion then shifts to the mechanisms of action of lymphokines in vitro and the use of animal models in lymphokine investigations, including lymphokine fractions in a mouse tumor model and enhancement of natural killing activity by different types of interferon. A great deal of emphasis is placed on macrophage migration inhibitory factor, lymphotoxins, gamma interferon, and interleukins. The book concludes with chapters devoted to the immunoregulatory effects of lymphokines and clinical studies.

ContributorsPrefaceAcknowledgmentsI. Assay Systems: Standardization and Nomenclature Polyclonal B Cell Activator in Rheumatic Diseases Interferon Activates Macrophages to Produce Plasminogen Activator A Standardized Automated Computer Assisted Micro-Assay for Lymphotoxin Problems Associated with Research on Human MIF The Motility of the Cells of a Lymphoblastoid Cell, QIMR-WIL, Is Inhibited by Human and Murine Leukocyte Inhibitory Factor A Standardized and Rapid MIF Assay Using U-937 Cell Line and Optical Density Measurement Macrophage Activation by Lymphokine Factors (MAF) as Measured by Phagocytosis, Metabolic Products, and Tumor Cell Cytotoxicity: Correlation of Multiparameter Functional Assays Standardization of the Indirect LIF Test by Reference to Lymphoid Cell-Line Lymphokine (LCL-LK) as a Standard Material Value of Reference Preparations in Lymphokine Tests Semimicromodification of the Indirect Migration Inhibition / MIF Production / TestII. Production And Purification Induction and Augmentation of Mitogen-Induced Lymphokine Production in Human PBL by Na-Desacetylthymosin a1 Production of Human Interleukin-2(TCFG) Using Serum Free Culture Conditions Morphological Characteristics of Interferon-Producing Cells in Human Peripheral Blood Rapid Production of High Levels of Gamma Interferon by Human Thoracic Duct Leukocytes Stimulated with Staphylococcal Enterotoxin A Gamma (Immune) Interferon Production by a Permanent Human T-Lymphocyte Cell Line Purification of Lymphotoxin from RPMI-1788 Cell Line Supernate The Identification of Two New Classes of Lymphotoxin Released by Lectin Activated Human T Lymphocytes that Possess IG-Like Receptors Purification of the Alpha-Heavy Class of Human Lymphotoxin to Electrophoretic Homogeneity Production of Leucocyte Migration Inhibitory Factor (LIF) by Human Natural Killer (NK) Cells Spontaneous Production of Leucocyte Migration Inhibitory Factor (LIF) by Established Human Lymphoid Cell Lines Lymphokine Induced Release of Basophilic Histamine: Separation of the Activity into Two Factors The Physiological Interferon Response: The Sites and the Meaning Interferons Induced in Human Leukocytes by Mitogens: Production, Partial Purification, and CharacterizationIII. Biological and Chemical Characterization Human Interleukin 1: Purification and Properties Human (T, G)-A-L Specific Helper Factors Possess VH as Well as HLA-DR Determinants Production and Characterization of Human Gamma Interferon Human Lymphocyte Production to Neuroendocrine Hormone-Related Substances Neutrophil Migration Inhibition Factor from T-Lymphocytes (NIF-T): Specific Binding to Human Peripheral Blood Neutrophils and Neutrophil Precursors Production and Biological Characterisation of Lymphoid Cell-Line Lymphokine (LCL-LK) Preparations Macrophage Agglutination Activity of Human Plasma Proteins Purification of Human Lymphokines by High Performance Liquid Chromatography Human Serum Factor Inducing Giant Fat Cell Formation in Human Bone Marrow Culture: Normal and Leukemic Human Leukocyte Interferon Comprises a Continuum ofIV.