Methods of Animal Experimentation

Methods of Animal Experimentation, Volume III, compiles methods of animal experimentation in a variety of fields so that the researcher might have a reference that would allow him to incorporate other techniques in his research studies. The methods described represent only the most efficient ways known to date for using animals to gain research information. They are not, however, ""standard methods"" for research in which each experiment seeks or confirms new information by another means. The largest portion of this volume is devoted to behavioral science because of the growing importance of this field in two respects. First, it is an important consideration in all experiments in which animals are used repeatedly. Second, the use of higher animals in comparative behavioral research offers much toward the solution of disease problems of man related to behavior and perhaps even clues to his social interactions. A chapter by an author with extensive experiences with dolphins helps us judge how this animal may be used, and warns of the many problems incurred in research using any of the marine mammals. Other chapters cover dental research on animals, fetal surgery and physiological measurements in very young animals, and microsurgery.

List of ContributorsPrefaceContents of Previous VolumesChapter 1 bUse of Rodents in Behavioral Research I. Introduction II. The Physical Environment III. Basic Concepts IV. Preexperimental Preparations V. Behavioral Control ReferencesChapter 2 Use of the Dog in Behavioral Research I. Introduction II. General Procedures in Dog Testing III. Specific Tests and Observations IV. Conditioning Procedures V. Spontaneous Behavioral Abnormalities in Dogs References General ReferencesChapter 3 Use of Primates as Behavioral Research Subjects I. Introduction II. Response Classification III. Experimental Problems Often Studied IV. Apparatus and Concepts of Equipment Use V. Maintenance of Primates for Behavioral Studies ReferencesChapter 4 Use of Cats in Behavioral Research I. Introduction II. Behavioral Development III. Comparative Studies IV. Assessment Studies V. Conclusions ReferencesChapter 5 Microsurgery I. Introduction II. Equipment for Microsurgery III. Principles of Microsuture Techniques and Other Manipulations IV. Microsurgery of Vessels Supplying Extremities, Brain, Heart, and Other Organs V. Microsurgery of Nerves VI. Microtechniques in Ophthalmology VII. Microtechniques in Otology and Laryngology VIII. Microtechniques in Surgery and Gynecology IX. Microtechniques in Neurosurgery and Experimental Neurology X. Clinical Application, Transplantation of Organs, and General Aspects of Further Development ReferencesChapter 6 Experimental Procedures and Surgical Techniques Performed on Intrauterine Fetal Animals I. Introduction II. Preparation of the Pregnant Animal III. Closed Abdominal Preparation IV. Closed Uterine Preparation V. Closed Fetal Membrane Preparation VI. Uterine Window Preparation VII. Modified Uterine Window Preparation VIII. Exteriorization of the Fetus IX. Thymectomy X. Catheterization Techniques XI. Splenectomy ReferencesChapter 7 Physiological Measurements in Neonatal Animals I. Introduction II. General Considerations III. Removal of Nervous and Other Tissues IV. Electrophysiological Measurements from the Developing Brain V. Anesthetic VI. Implantation of Electrodies for Physiological Recordings VII. Postoperative Care VIII. Conditions for Recording IX. Administration of Drugs X. Special Considerations for Studies of Fetal Animals ReferencesChapter 8 The Use of Animals in Dental Research I. Introduction II. Characteristics of Various Animal Species III. Housing and Management IV. Dental Diseases V. Transplantation, Replantation, and Implantation ReferencesChapter 9 Methods of Using Fish as Biological Research Subjects I. Introduction II. Maintaining Fish in the Laboratory III. Anesthesia IV. Surgical Manipulations V. Blood and Vascular Function VI. Renal Function VII. Locomotor Function VIII. Gill Function IX. Gastrointestinal Functions X. Neurosensory Functions XI. Hormone Function ReferencesChapter 10 The Bottlenosed Dolphin in Biomedical Research I. Introduction II. Anatomy III. Senses IV. Physiology V. Clinical Procedures VI. Behavior and Husbandry VII. Disease Problems and Therapeutics VIII. Clinical Observation of Behavior IX. Scope and Limitations of Tursiops in Biomedical Experimentation ReferencesAuthor Index Subject Index