Handbook of Histopathological and Histochemical Techniques

Handbook of Histopathological and Histochemical Techniques (Including Museum Techniques), Third Edition is a reference guide on techniques used in histology and histochemistry, including museum techniques. This book is comprised of 36 chapters divided into six sections. After giving a brief description of the structure and contents of the cell, this book then turns to the various methods of examination of tissue cells and structures, including the smear method, vital staining, and electron microscopy. The next two sections deal with fixation, decalcification, processing, and section cutting, along with stains, impregnations, and mountants. An exhaustive survey of carbohydrate and protein histochemistry is included. Special procedures such as autoradiography, micro-incineration, and injection techniques are described. The remaining chapters focus on the preparation, mounting, and presentation of museum specimens. The book concludes with an analysis of the microscope. This monograph will be invaluable to all those with an interest in histology and histochemistry, including undergraduate students in the biological and health sciences, pathologists in both the hospital setting and experimental research, and technologists and workers in various research fields.

ForewordPreface to Third EditionPreface to First EditionAcknowledgmentsPart I Introductory Chapter 1. The Cell Chapter 2. Methods of Examination of Tissues and CellsPart II Fixation, Processing and Section Cutting Chapter 3. Fixation Chapter 4. Decalcification Chapter 5. Processing Chapter 6. Section CuttingPart III Stains, Impregnations and Mountants Chapter 7. Theory of Staining Chapter 8. Preparation of Stains Chapter 9. Mountants Chapter 10. Basic Staining and Mounting Procedures Chapter 11. Routine Stains Chapter 12. Proteins Chapter 13. Nucleoproteins-Deoxyribonucleic Acid and Ribonucleic Acid Chapter 14. Carbohydrates Chapter 15. Enzymes Chapter 16. Lipids (Fats, Lipoids, Lipins) Chapter 17. Pigments Chapter 18. Micro-Organisms Chapter 19. Tissues Requiring Special Treatment or Techniques Chapter 20. Certain Cytoplasmic Constituents and Cell ProductsPart IV Special Procedures Chapter 21. Exfoliative Cytology and Chromosome Techniques Chapter 22. Autoradiography Chapter 23. Vital Staining Chapter 24. Micro-Incineration Chapter 25. Injection TechniquesPart V Museum Technique Chapter 26. Preparation, Color Maintenance, Fixation and Storage of Specimens Chapter 27. Mounting of Museum Specimens Chapter 28. Special Methods Chapter 29. Presentation of Museum SpecimensPart VI The Microscope Chapter 30. The Compound Microscope Chapter 31. The Dark-Ground Microscope Chapter 32. Fluorescence Microscopy Chapter 33. The Polarizing Microscope Chapter 34. The Phase-Contrast Microscope Chapter 35. The Interference Microscope Chapter 36. The Electron MicroscopeReferencesIndex