Organization and Practice in Tuberculosis Bacteriology

Organization and Practice in Tuberculosis Bacteriology focuses on the organization and technical methods of tuberculosis bacteriology. This book discusses the mycobacteria and mycobacterial disease and role and scope of the tuberculosis laboratory in human and veterinary medicine and epidemiology. The organization of tuberculosis laboratory services; collection, preservation and transport of specimens; and examination of direct smears are also elaborated. This text likewise covers the decontamination of specimens, inoculation of culture media, and identification of species and variants of tubercle bacilli. Other topics include the drug sensitivity tests, animal inoculation and assay tests; problems faced in the therapy of tuberculosis, and lipid chromatography. This publication is suitable for biologists, medical practitioners, and clinicians concerned with tuberculosis bacteriology.

1 Introduction: Mycobacteria and Mycobacterial Disease Tuberculosis Other Mycobacterioses in Man2 The Role and Scope of the Tuberculosis Laboratory in Human and Veterinary Medicine and Epidemiology3 The Organization of Tuberculosis Laboratory Services Grades of Laboratories Design of the Tuberculosis Laboratory Equipment Training and Motivation of Staff Proficiency Testing Prevention of Laboratory-Acquired Tuberculosis4 Collection, Preservation and Transport of Specimens Sputum Urines Aspirated Fluids and Pus Tissues Milk Samples Water Samples Identifying Specimens Preservation of Specimens Transport5 Examination of Direct Smears Preparation of Specimens Staining Quantitative Microscopy Precautions against False Positives Positive Smears - Negative Cultures Acid- and Alcohol-Fast Bacilli Storage and Disposal of Slides6 Cultural Methods Decontamination of Specimens Culture Media Inoculation of Culture Media Incubation Examination of Cultures Radiometrie Method for TB Culture7 Taxonomic Principles - The Basis of Identification8 Identification of Species and Variants of Tubercle Bacilli Nomenclature Identification Identification of Variants of M. Tuberculosis and BCG Variants and Species of Tubercle Bacilli9 Identification of 'Other Mycobacteria' Terminology Identification Methods Cultural and Biochemical Tests Lipid Chromatography Additional Biochemical Tests Additional Reference Techniques Species of Medical and Veterinary Interest Mycobacterium Avium Mycobacterium Avium-Intracellulare-Scrofulaceum '(MAIS)' Mycobacterium Chelonei Mycobacterium Flavescens Mycobacterium Fortuitum Mycobacterium Gastri Mycobacterium Gordonae Mycobacterium Haemophilum Mycobacterium Intracellulare Mycobacterium Kansasii Mycobacterium Malmoense Mycobacterium Marinum Mycobacterium Nonchromogenicum Mycobacterium Paratuberculosis Mycobacterium Phlei Mycobacterium Scrofulaceum Mycobacterium Simiae Mycobacterium Smegmatis Mycobacterium Szulgai Mycobacterium Terrae Mycobacterium Triviale Mycobacterium Ulcerans Mycobacterium Xenopi Species of Limited Interest in Human and Veterinary Medicine10 Drug Sensitivity Tests The theory of Drug Resistance and Treatment Regimens Principles of Treatment Regimens Development of Modern Treatment Regimens Problems Faced in the Therapy of Tuberculosis The Treatment of Other Mycobacterial Infections The Role of Sensitivity Testing Methods for Sensitivity Tests11 Animal Inoculation and Assay Tests Animal Inoculation Tests Assay of Antimicrobial Agents in Serum and their Detection in Urine12 Leprosy Technical Procedures The Skin The Nose BiopsiesAppendix Stains, Culture Media and Other MaterialsReferencesIndex