Cell Biology of the Major Histocompatibility Complex

Cell Biology of the Major Histocompatibility Complex documents the proceedings of a symposium on ""Cell Biology of the Major Histocompatibility Complex"" held at Arden House on the Harriman Campus of Columbia University from June 8 -10, 1984. The meeting was the ninth of the P & S Biomedical Sciences Symposia. The book is organized into five parts. Part I on the structure of MHC molecules includes papers on human histocompatibility antigens; the cloning of human MHC; and organization of the genes of the H-2 complex. Part II on alternate forms of MHC molecules includes studies on the expression of a secreted form of the MHC class I antigen and alternative splicing in the H-2 multigene family. Part III deals with MHC biosynthesis, intracellular transport, and membrane expression. It includes studies on the manipulation of glycans on antigens of the MHC and dynamics of MHC molecules in lymphoid cells. Part IV on homologues of class I MHC molecules covers topics such as Tla expression in normal and malignant cells and the Qa series of antigens. Part V on the association of MHC with antigens includes studies such as histocompatibility molecules as immune response gene products and molecular nature of T-cell recognition of antigen.

List of ParticipantsPrefaceOpening Address The Biological Significance of the Antigens of the Major Histocompatibility Complex Introduction The Selective Expansion of Thymocytes with Receptors for Self-Ia The Origin of Alloreactivity The Interaction between Class II MHC Molecules and Antigen: A Basis for the Specificity of Ir Gene-Controlled Phenomena ReferencesPart I. The Structure of MHC Molecules: Implications for Functions Human Histocompatibility Antigens: Genes and Proteins Text References New Approaches and Results in Cloning of the Human Major Histocompatibility Complex Text References Cloning at a Distance with a Circularization Technique Text References Mutants of the Murine Major Histocompatibility Complex: Structural Analysis of in Vivo and in Vitro H-2Kb Variants Introduction In Vivo MHC Mutants In Vitro MHC Mutants References Phylogeny of MHC Class I and Class II Molecules Identified by Cross-Reactive Xenoantisera Introduction Results and Discussion References Organization of the Genes of the H-2 Complex Introduction Genetic Map of the Major Histocompatibility Complex A Large Number of Class I Genes A Chromosome Walk in the I Region Variable or Conserved Chromosomal Tracts Future References Membrane Expression and Function of MHC Class I Antigens with Truncated or Altered Cytoplasmic Tails Strategies of in Vitro Mutagenesis Cytoplasmic Tail Sequences Required for Expression of a Functional MHC Molecule on the Cell Surface Does the Cytoplasmic Tail Have a Role in the Lateral Mobility of the MHC Molecule on the Cell Membrane? Effect of the Cytoplasmic Tail on the Internalization of the MHC Molecule Conclusions References A Gene Conversion Event in the I-Aßb Gene Alters the Self-I-A Restricted T-Cell Repertoire of B6.C-H-2bm12 Mice Introduction The Role of Ia Epitopes in Conferring Specificity for the Immune Response to Nominal Antigen Discussion References Evolution of H-2 Genes on t Chromosomes Text ReferencesPart II. Alternate Forms of MHC Molecules Expression of a Secreted Form of the MHC Class I Antigen Text References Alternative Splicing in the H-2 Multigene Family Introduction The pH-2d-33 and pH-2d-24 cDNA Clones Demonstration of Alternate Splicing in the The "33" and "24" Transcripts Are Made in a Variety of Tissues of DBA/2 Mice Alternative Splicing in the Alternative Splicing in the Q10 Gene Alternative Splicing in Other Class I Genes Evolution of Class I Genes through Mutations Altering Splicing Events Potential for Alternative Splicing in Class I Genes What Determines Alternate Splicing? Possible Significance of Alternative Splicing ReferencesPart III.