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Chromatographic and Electrophoretic Techniques, Volume I - Chromatography focuses on techniques, processes, reactions, and methodologies involved in chromatography. The selection first ponders on paper chromatographic apparatus and techniques; desalting and related techniques; and apparatus and techniques in thin layer chromatography. Discussions focus on chromatographic solvents, location reagents, chemical conversions occurring during electrolytic desalting, electrodialysis, and ion exchange desalting. The book also examines paper chromatography, applications of thin layer chromatography in clinical biochemistry, and dinitro-phenyl aminoacids. The publication takes a look at iodoaminoacids and related compounds, indoles and related Ehrlich reactors, and imidazoles. The book also elaborates on guanidines, purines and pyrimidines and their derivatives, sugars, ketoacids, organic and phenolic acids, and chromatographic procedures. The selection is a dependable reference for biochemists and readers interested in chromatography.
1. Introduction to Paper and Thin Layer Chromatography2. Paper Chromatographic Apparatus and Techniques3. Desalting and Related Techniques4. Thin Layer Chromatography (TLC) Section 1 - Apparatus and Techniques Section 2 - Pre-Coated Plates Section 3 - Non-Adhering Layers5. Aminoacids, Amines and Related Compounds The Pathological Aminoacidurias6. Dinitro-Phenyl Aminoacids7. Aminoacid Phenylthiohydantoins, Dimethylaminonaphthalenesulphonyl Aminoacids and Sequence Analysis of Peptides and Proteins8. Iodoaminoacids and Related Compounds9. Indoles and Related Ehrlich Reactors10. Imidazoles11. Guanidines12. Purines, Pyrimidines and Their Derivatives13. Sugars and Related Compounds14. Ketoacids15. Organic Acids of the Citric Acid Cycle and Related Acids16. Phenolic Acids17. Plant Phenols and Tannins18. Lipids19. Separation and Estimation of Phospholipids20. Barbiturates21. Alkaloids22. Glutarimides and Other Neutral Drugs23. Steroids24. Rm and -Rm: Making Full Use of Rf Values25. Chromatography of Radioactive Substances26.Chromatography of Labeled Cell Extracts27. The Spark Chamber Technique for Radio-Chromatograms28. Catecholamlnes, Their Precursors and Metabolites29. Ion-Exchange Celluloses in Chromatography30. Amino Sugars31. Kynurenine Pathway Metabolites of Tryptophan32. Inorganic Ions33. Sephadex Thin-Layer Gel Filtration34. Preparative Layer Chromatography35. Automated Quantitative Analysis of Paper Chromatogram Strips36. Pigments The Forensic Examination of Inks pH Indicators, Anthocyanins and Pteridines37. Column Chromatographic Apparatus and Techniques38. The Investigation of New Compounds39. Fractionation and Sequence Analysis of [32p]. Labeled Ribonucleotides40. Modelsformacromolecules, Nucleic Acids and ProteinsGeneral Index