Colon Cancer Cells

Colon Cancer Cells brings together fundamental research and clinically relevant issues in the cell biology of colon cancer. This book is composed of five parts encompassing 21 chapters that specifically describe the initiation and progression of colon cancer cells. After briefly dealing with the major issues in colorectal carcinoma, this book goes on presenting the in vitro and in vivo models of colon carcinogenesis. This topic is followed by a discussion on the history of the development and characterization of commonly used colon cancer cell lines. The following parts describe the biochemical and immunological features and hormones in the colon. These parts also consider the studies on human colon tumors xenografted into nude mice and the biology and treatment of colorectal cancer metastasis. Discussions on the application of human monoclonal antibodies to tumor detection; the expression of blood group-related carbohydrates by normal, premalignant, and malignant colonic tissues; and the correlation of antigen variability in colon carcinoma with certain diagnostic and prognostic parameters are also included in these parts. The concluding part examines various therapeutic strategies and their potential in improving patient management with advanced colon carcinoma. Researchers, clinicians, and students interested in the biology of colon cancer development and in gastrointestinal cell biology will find this book invaluable.

Contributors Preface 1 Colorectal Carcinoma: Key Issues Text References I. Normal, Preneoplastic, and Neoplastic Colonic Epithelium In Vivo and In Vitro 2 Normal and Pathological Anatomy of the Large Intestine I. Introduction II. Normal Large Intestine III. Precursors of Carcinomas IV. Carcinoma of the Large Intestine References 3 Kinetics of Normal, Preneoplastic, and Neoplastic Colonic Epithelium I. Introduction II. General Principles III. Normal Colon IV. Preneoplastic Conditions V. Neoplasia References 4 Colon Carcinogenesis: Modulation of Progression I. Introduction II. Multistep Model of Carcinogenesis III. Cell Proliferation and Colon Carcinogenesis IV. Attempts to Modify Cell Proliferation as a Means of Modulating Progression of Colon Carcinogenesis with Special Reference to Dietary Fiber V. Summary and Conclusions References 5 In Vitro Propagation and Characterization of Normal, Preneoplastic, and Neoplastic Colonic Epithelial Cells I. Introduction II. Development of Culture Methods III. Characterization of Cultured Cells IV. New Avenues of Research V. Conclusions References 6 Colon Organ Culture as a Model for Carcinogenesis I. Introduction II. Techniques III. Applications IV. Carcinogenesis Studies V. Discussion References 7 Human Cell Lines in Colon Cancer Research I. Introduction II. Origins of Cell Lines III. Methods and Success of Culture Initiation IV. Growth and Morphology Characteristics V. Differentiation and Genetic Markers VI. Tumorigenicity VII. Other Phenotypic Features VIII. Conclusions References 8 Preclinical Evaluation of Cancer Chemotherapeutic Drugs for Treatment of Colorectal Cancer I. Introduction II. Recent Advances in Experimental Chemotherapy of Human Colorectal Cancer III. Screening System Based on Use of Human Colorectal Cancer Cell Lines IV. Conclusions References II. Regulation of Normal and Colon Cancer Cells 9 Characteristics of Human Colorectal Cell Lines Established in Defined and Serum-Supplemented Media I. Introduction II. Culture Methods III. Phenotypic Variation IV. Antigen Expression V. Neuroendocrine Differentiation VI. Other Findings VII. Comparative Properties of SSM- and ACL-4-Derived Cell Lines VIII. Conclusions References 10 Growth Factors I. Morphologic and Chemical Markers of Colonic Cell Proliferation II. Postoperative Colon Adaptation in Animals and Humans III. Luminal Factors IV. Studies of Tropic Hormones In Vivo V. In Vitro Studies of Hormones and Growth Factors VI. Studies of Carcinogenesis In Vitro VII. Intracellular Mechanisms of Cell Proliferation VIII. Summary References 11 Polyamines and Colon Cancer Cells I. Introduction II. Ornithine Decarboxylase and Polyamines III. Polyamines and Intestinal and Colonie Mucosal Growth IV. Polyamines and Chemical Colonic Carcinogenesis V. Polyamines and Human Colon Cancer Cell Growth VI. Polyamines and Human Colon Cancer Xenografts VII. Polyamines as Markers of Colon Cancer VIII. Summary References 12 Current Status of Colon Cancer Cytogenetics I. Introduction II. Background III. Chromosome Changes in Colon Adenomas (Polyps) IV. Chromosome Changes in Colon Cancer V. Colon Cancer Cell Lines VI.