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High-Resolution Electrophoresis and Immunofixation: Techniques and Interpretation acquaints the reader with the available methodologies and instrumentation for performing high-resolution electrophoresis and immunofixation. This text presents the use of procedures of high-resolution electrophoresis and immunofixation to facilitate the diagnosis of a variety of serum protein abnormalities. It also provides case examples to illustrate the uses of these procedures and their possible caveats. The book contains chapters devoted to the methods and rationale for high-resolution electrophoresis; interpretation of high-resolution electrophoresis patterns in serum, urine, and cerebrospinal fluid; the immunofixation technique; and strategies for diagnosing monoclonal gammopathies. Medical technologists, physicians, and pathologists will find the book invaluable.
Preface1. Methods and Rationale for High-Resolution Electrophoresis Review of Protein Structure and Electrophoretic Techniques Methods of High-Resolution Electrophoresis High-Resolution Electrophoresis versus Standard Electrophoresis References2. Serum Proteins Identified by High-Resolution Electrophoresis Proteins Identified by High-Resolution Electrophoresis Alpha Region Inter-Alpha-1, -Alpha-2 Region Beta Region Gamma Region References3. Interpretation of High-Resolution Electrophoresis Patterns in Serum, Urine, and Cerebrospinal Fluid Approach to Pattern Interpretation Interpretation of the Individual Patient's Sample Pattern Interpretation in Cerebrospinal Fluid HRE Interpretation for Urine References4. Immunofixation Technique Principles of Immunoprecipitation Immunoelectrophoresis Limitations of Immunoelectrophoresis Immunofixation Limitations of Immunofixation Cost Considerations References5. Strategies for Diagnosing Monoclonal Gammopathies Ontogeny of B Lymphocytes T-Lymphocyte Development Conditions Associated with Monoclonal Gammopathies Monoclonal Gammopathies in Patients with B-Lymphocyte Neoplasms Laboratory Diagnosis of Monoclonal Gammopathies References6. Case Studies for Interpretation General Comments about InterpretationIndex
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