Methods in Mycoplasmology V2

Methods in Mycoplasmology, Volume II: Diagnostic Mycoplasmology is the second of a two-volume series that resulted from cumulative efforts to meet the need for standardized techniques in mycoplasmology. The book is organized into five sections. Section A discusses techniques for isolating mycoplasmas from humans. These include the recovery of mycoplasmas from the respiratory and genitourinary tracts, from blood and special tissues, and serological Identification of mycoplasmas from humans. Section B focuses on techniques for isolating mycoplasmas from animals, including the recovery and identification of avian, bovine, caprine, ovine, canine, and feline mycoplasmas. Section C presents techniques for isolating mycoplasmas from cell cultures. These include agar and broth techniques, the use of indicator cell lines, and DNA fluorochrome staining. Section D discusses techniques for isolating mycoplasmas from plants and insects. Section E describes some of the techniques that are useful in evaluating the pathogenicity of mycoplasmas, ureaplasmas, and spiroplasmas. Section F defines some of the techniques useful in virus detection by electron microscopy and presents methods for isolating and characterizing viruses from both mycoplasmas and spiroplasmas.

ContributorsPrefaceContents of Volume ISection A Special Techniques for Isolation and Identification of Mycoplasmas from Humans A1 Introductory Remarks A2 Recovery of Mycoplasmas from the Respiratory Tract A3 Recovery of Mycoplasmas from the Genitourinary Tract A4 Recovery of Mycoplasmas from Blood and Special Tissues A5 Serological Identification of Mycoplasmas from Humans A6 The Complement Fixation Test for Diagnosis of Mycoplasma pneumoniae Infection A7 Serological Identification of Ureaplasmas from HumansSection - Special Techniques for Isolation and Identification of Mycoplasmas from Animals B1 Introductory Remarks B2 Recovery and Identification of Avian Mycoplasmas B3 Recovery and Identification of Bovine Mycoplasmas B4 Recovery and Identification of Caprine and Ovine Mycoplasmas B5 Recovery and Identification of Canine and Feline Mycoplasmas B6 Recovery of. Identification of, and Serological Response to Porcine Mycoplasmas B7 Recovery and Identification of Murine Mycoplasmas B8 Recovery and Identification of Mycoplasmas from Other Laboratory Animals (Including Primates) B9 Lavage Techniques for Recovery of Animal MycoplasmasSection C Special Techniques for Isolation and Identification of Mycoplasmas from Cell Cultures C1 Introductory Remarks C2 Isolation of Mycoplasmas from Cell Cultures by Agar and Broth Techniques C3 Use of Indicator Cell Lines for Recovery and Identification of Cell Culture Mycoplasmas C4 Detection and Identification of Mycoplasmas in Infected Cell Cultures by Direct Immunofluorescence Staining C5 Detection of Mycoplasmal Infection of Cell Cultures by DNA Fluorochrome Staining C6 Biochemical Procedures for the Detection of Mycoplasmal Infection in Cell Cultures C7 Prevention and Control of Mycoplasmal Infection of Cell CulturesSection D Special Techniques for Isolation and Identification of Mycoplasmas from Plants and Insects D1 Introductory Remarks D2 Culture Techniques for Spiroplasmas from Arthropods D3 Culture Techniques for Spiroplasmas from Plants D4 Culture Techniques for Plant Mycoplasmas D5 Physical Techniques for Purification of Mycoplasmas from Plant Tissues D6 Special Serological Tests for Spiroplasma Identification D7 Transmission Techniques with Vectors of Plant and Insect Mycoplasmas and Spiroplasmas D8 Antibiotic Treatment of Plants and Bioassay to Determine Foliar Antibiotic Concentration D9 Treatment of Woody Plants with AntibioticsSection - Pathogenicity Tests and Antibiotic Sensitivity Testing E1 Introductory Remarks E2 Respiratory Challenge of Experimental Animals E3 Intramammary Inoculation of Cattle and Other Animals E4 Evaluation of Arthritogenic Properties of Mycoplasmas for Small Laboratory Animals E5 Tests for Mycoplasma neurolyticum Neurotoxin E6 Pathogenicity Tests in Cell Cultures E7 Genitourinary Challenge in Various Hosts E8 Tracheal Organ Cultures as Models in Pathogenicity Studies E9 Fallopian Tube Cultures as Models in Pathogenicity Studies E10 Tests for Adherence Properties of Mycoplasmas E11 Demonstration of Phytotoxin Production by Spiroplasmas E12 Pathogenicity of Mycoplasmas for Arthropods and Its Possible Significance in Biological Control E13 Special Procedures for Demonstration of Mycoplasma Pathogenicity in Insects E14 Determination of Spiroplasma Pathogenicity by Inoculation of Suckling Rats and Embryonated Chicken Eggs E15 Assays for Lymphocyte Activation E16 Antibiotic Sensitivity Testing of MycoplasmasSection F Mycoplasma Viruses F1 Introductory Remarks F2 Virus Detection by Electron Microscopy F3 Isolation and Characterization of Mycoplasma Viruses F4 MV-L2: Growth and Purification F5 Isolation and Characterization of Spiroplasma VirusesIndex