Pathology, Vector Studies, and Culture

Malaria: Volume 2, Pathology, Vector Studies, and Culture is a collection of papers that deals with erythrocyte destruction mechanism in malaria, the pathology of malaria, colonization of laboratory mosquitoes, and their transmission of plasmodia. Other papers describe the culture of the invertebrate stages of plasmodia, of mosquito tissues, and also of erythrocytic and exoerythrocytic stages of plasmodia. One paper constructs a model to show the roles of the different destructive and regenerative processes in the mechanisms of erythrocyte destruction in malaria. Another paper describes the organ changes and physiopathological mechanism connected with Plasmodium infection. These organs concern the spleen, liver, heart, and vascular system. One paper reviews the procedures involved in malaria transmission by mosquitoes, as well as some specialized procedures unique in avian, rodent, simian, and human malarias. Another paper discusses the benefits and drawbacks of a culture medium based on the composition of adult anopheline hemolymph. This medium should lead to a rapid growth rate in primary cultures of mosquito cells and a shorter interval of adaptation for continuous cell lines. The collection can prove useful for pharmacologists, general medical practitioners, investigators, and laboratory technicians involved in mosquito borne diseases or tropical medicine research.

-List of ContributorsPrefaceContents of Volumes 1 and 3Contents of Babesiosis1 Erythrocyte Destruction Mechanisms in Malaria I. Introduction II. Mechanisms of Erythrocyte Destruction III. Proposed Model of the Relationship between Erythrocyte Loss and Parasitemia References2 Pathology of Malaria I. Introduction II. Spleen Pathology III. Liver Pathology IV. Heart and Vascular Pathology V. Hematopathology VI. Pulmonary Pathology VII. Thymus Pathology VIII. Renal Pathology IX. Neuropathology X. Placenta Pathology References3 Colonization and Maintenance of Mosquitoes in the Laboratory I. Introduction II. Basic Mosquito Biology III. Collection of Field Material IV. Transport of Living Material V. Rearing VI. Selection Procedures VII. Pathogen and Pesticide Problems References4 The Transmission by Mosquitoes of Plasmodia in the Laboratory I. Introduction II. Susceptibility of Mosquitoes to Plasmodium III. Gametocytes in the Vertebrate Host IV. The Infective Blood Meal V. Maintenance of Infected Mosquitoes VI. Sampling Procedures for Determining the Degree of Mosquito Infection VII. Harvesting, Purifying, and Maintaining Sporozoites VIII. Infecting Vertebrates with Sporozoites IX. Quantitation of Sporozoite Infectivity and Viability X. Specific Host-Parasite-Vector Systems in Malaria Research XI. Conclusions References5 Culture of the Invertebrate Stages of Plasmodia and the Culture of Mosquito Tissues I. Introduction II. Attempts to Culture the Vertebrate Stages of Plasmodia III. Current Methods of Initiating Primary Cell and Organ Cultures from Mosquito Species with Emphasis on Anophelines IV. Adult Anopheline Hemolymph Analysis and the Design of Culture Media V. Concluding Remarks References6 Cultivation of Erythrocytic and Exoerythrocytic Stages of Plasmodia I. Introduction II. Culture of Erythrocytic Stages within Their Host Cells III. Extracellular Culture of Erythrocytic Stages IV. Exoerythrocytic Stages in Culture ReferencesIndex