Providing Pharmacological Access to the Brain

This volume focuses on contemporary approaches for delivering experimental and therapeutic agents into the brain. The contributions provide methodological details that are typically not available in the literature. Subtleties and shortcuts critical to each procedure are included to facilitate their use by both the experienced researcher and novice.Highlights* Polymeric, cellular, and molecular drug delivery* Neuropharmacology* Blood-brain barrier* Central nervous system

Characterizing the Blood-Brain Barrier:A. Logan and M. Berry, Cellular Response of Central Nervous System Tissue to Invasive Therapeutic Measures.J.M. Rosenstein and J.M. Krum, Models of Angiogenesis and the Blood-Brain Barrier.Transiently Removing the Blood-Brain Barrier:P.J. Robinson, Osmotic Opening of the Blood-Brain Barrier and Brain Tumor Chemotherapy.E.A. Neuwelt and R.A. Kroll, Osmotic Blood-Brain Barrier Modification: Increasing Delivery of Diagnostic and Therapeutic Agents to the Brain.Facilitated Transport through the Blood-Brain Barrier:A.-C. Granholm, P.T. Biddle, C. Bäckman, T. Ebendal, G. Gerhardt, B. Hoffer, L. Mackerlova, L. Olson, S. Söderström, L. Walus, and P. Friden, Peripheral Administration of Nerve Growth Factor Conjugated to an Anti-transferrin Receptor Antibody Increases Cholinergic Neuron Survival in Intraocular Forebrain Transplants.R.D. Broadwell, B.J. Baker, W.A. Banks, P. Friden, M. Moran,C. Oliver, and J.C. Villegas, Ferrotransferrin and Antibody against the Transferrin Receptor as Potential Vehicles for Drug Delivery across the Mammalian Blood-Brain-Barrier into the Central Nervous System.S. Kim, Depofoam-Mediated Drug Delivery into Cerebrospinal Fluid. Polymeric Release Systems for the Central Nervous System:R.J. Tamargo, R. Langer, and H. Brem, Interstitial Drug Delivery to the Central Nervous System Using Controlled Release Polymers: Chemotherapy for Brain Tumors.A. Mendez, P.J. Camarata, R. Suryanarayanan, and T.J. Ebner, Sustained Intracerebral Delivery of Nerve Growth Factor with Biodegradable Polymer Microspheres.A.J. Domb and I. Ringel, Polymeric Drug Carrier Systems in the Brain.Using Pump Delivery Devices within the Brain:J.D. White and M.W. Schwartz, Using Osmotic Minipumps for Intracranial Delivery of Amino Acids and Peptides.T. Hagg, Continuous Central Nervous System Infusion with Alzet Osmotic Pumps.P.M. Carvey,T.J. Maag, and D. Lin, Injection of Biologically Active Substances into the Brain.Using Implanted Living Tissues within the Brain:R. Barker, R. Fricker, and S.B. Dunnett, Factors Important in the Survival of Dopamine Neurons in Intracerebral Grafts of Embryonic Substantia Nigra.M.S. Fiandaca and J.H. Kordower, Techniques in Adrenal Medullary Transplantation for Experimental Nonhuman Primate Parkinsonism.S.W. Carmichael, S.L. Stoddard, and P.J. Kelly, Technical Aspects of Transplantation of the Adrenal Medulla to the Caudate Nucleus as a Treatment for Parkinsons Disease.Creating Cell Lines for Transplant Therapies: J.P. Hammang, B.A. Reynolds, S. Weiss, A. Messing, and I.D. Duncan, Transplantation of Epidermal Growth Factor-Responsive Neural Stem Cell Progeny into the Murine Central Nervous System.J.P. Kesslak and R.J. Bridges, Application of Astrocyte Transplants as a Therapeutic Intervention.M. Giordano, H. Takashima, M. Poltorak, H.M. Geller, and W.J. Freed, Development of Immortalized Cell Lines for Transplantation in Central Nervous System Injury and Degeneration Models.Using Implanted Living Cells within the Central Nervous System:L.J. Fisher, G.R. Chalmers, and F.H. Gage, Use of Genetically Modified Cells to Deliver Neurotrophic Factors and Neurotransmitters to the Brain.J. Sagen, J.D. Ortega, and G.D. Pappas, Neuropeptide and Catecholamine Delivery to the Central Nervous System by Implanted Chromaffin Cells.Using Implanted Encapsulated Cells within the Brain:M.V. Sefton, H. Uludag, J. Babensee, T. Roberts, V. Horvath, and U. De Boni, Microencapsulation of Cells in Thermoplastic Copolymer (Hydroxyethyl Methacrylate-Methyl Methacrylate).S.R. Winn and P.A. Tresco, Hydrogel Applications for Encapsulated Cellular Transplants.T.R. Flanagan, B. Frydel, B. Tente, M. Lavoie, E. Doherty, D. Rein, D.F. Emerich, and S.R. Winn, Test for Validating the Safety of Encapsulated Xenografts.Induced Gene Expression in Intrinsic Central Nervous System Cells with DNA Injected into the Brain:N.-S. Yang, S. Jiao, and C.