Research Methods in Biomineralization Science

This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in biomineralization science, and includes sections on such topics as determining solution chemistry, structure and nucleation; probing structure and dynamics at surfaces; and interfaces mapping biomineral and morphology and ultrastructure.
- Continues the legacy of this premier serial with quality chapters authored by leaders in the field
- Covers research methods in biomineralization science
- Contains sections on such topics as and includes sections on such topics as determining solution chemistry, structure and nucleation; probing structure and dynamics at surfaces; and interfaces mapping biomineral and morphology and ultrastructure

Determining solution chemistry, structure and nucleation1. Development of accurate force fields for the simulation of biomineralisation2. The integration of ion potentiometric measurements with chemical, structural and morphological analysis to follow mineralization reactions in solution.3. Investigating the Early Stages of Mineral Precipitation by Potentiometric Titration and Analytical Ultracentrifugation4. Replica Exchange Methods in Biomineral Simulations5. SAXS in inorganic and bio-inspired research6. In situ Solution Study of Calcium Phosphate Crystallization Kinetics Probing structure and dynamics at surfaces and interfaces7. Design, fabrication, and applications of in situ fluid cell TEM to mineralization reactions8. X-ray Absorption Spectroscopy for the Structural Investigation of Self-Assembled-Monolayer-Directed Mineralization9. Cryo-TEM Analysis of Collagen Fibrillar Stucture Biomimetic crystallization techniques in vitro and in silico10. Preparation of organothiol self-assembled monolayers for use in templated crystallization11. Experimental techniques for the growth and characterization of silica biomorphs and silica gardens 12. Precipitation in liposomes as a model for intracellular biomineralization13. Polymer Mediated Growth of Crystals and Mesocrystals14. Phage display for the discovery of hydroxyapatite associated peptides Protein structure, interactions and function15. Quantitatively and Kinetically Identifying Binding Motifs of Amelogenin Proteins to Mineral Crystals through Biochemical and Spectroscopic Assays16. Using the RosettaSurface Algorithm to Predict Protein Structure at Mineral Surfaces17. Investigating Protein Function in Biomineralized Tissues using Molecular Biology Techniques Mapping biomineral morphology and ultrastructure18. Imaging the nanostructure of bone and dentin through small- and wide-angle scattering19. Synchrotron X-ray Nanomechanical Imaging of Mineralized Fibre Composites Mapping mineral chemistry20. Application of Total X-ray Scattering Methods and Pair Distribution Function Analysis for Study of Structure of Biominerals21. X-ray Microdiffraction of Biominerals22. FT-IR and Raman studies of structure and bonding in mineral and organic-mineral composites23. A mixed flow reactor method to synthesize amorphous calcium carbonate under controlled chemical conditions