Standard Methods of Clinical Chemistry

Standard Methods of Clinical Chemistry, Volume 7 presents the methods to determine how an automated or radioisotope procedure can be best studied and evaluated. This book deals with subjects on control systems and standardization that are essential for effective operation of any clinical chemistry laboratory. Organized into seven parts encompassing 23 chapters, this volume begins with an overview of the technical aspects of the muramidase assay and its usefulness in the diagnosis of a variety of hematologic and renal disorders. This text then examines the use of olive oil as a substrate for measuring lipase activity. Other chapters consider the increased interest in the relationship of serum lipid fractions to coronary artery disease and the hyperlipoproteinemias. This book discusses as well the manual method for determination of serum iron. The final chapter deals with precipitating antigen-antibody systems used in diverse areas as immunology, microbiology, biochemistry, and forensic medicine. This book is a valuable resource for clinical chemists.

Miriam Reiner-A Biographical SketchContributorsPrefaceI. Enzymes Measurement of 5'-Nucleotidase Activity in Serum Assay of Muramidase Activity in Serum, Plasma, or Urine Measurement of Lipase Activity in Serum Measurement of Creatine Kinase Activity in Serum Colorimetric Determination of Lactate Dehydrogenase (L-Lactate : Nad Oxidoreductase) Activity LDH IsoenzymesII. Lipids Measurement of Phospholipids in Serum Semiautomated Fluorometric Measurement of Triglycerides Classification of Hyperlipidaemias and Hyperlipoproteinaemias Agarose Gel Electrophoresis of LipoproteinIII. Minerals, Heavy Metals Measurement of Serum Iron and Total Iron-Binding Capacity: Manual and Automated Techniques Total Calcium in Serum by Atomic Absorption Spectrophotometry Measurement of Lead in Blood, Urine, and Scalp Hair by Atomic Absorption SpectrophotometryIV. Proteins Technique of Immunoelectrophoresis Determination of Serum Albumin Histidine Procedure for Serum Protein Determinations with a Triphosphate Biuret ReagentV. Toxicology Ethchlorvynol ("Placidyl"): Determination in Serum and UrineVI. Vitamins and Hormones Vitamin A and Carotene Catecholamines and Associated Metabolites in Human UrineAssay of Serum Thyroxine I. Competitive Protein-Binding Radioassay of Serum ThyroxineAssay of Serum Thyroxine II. Total Thyroxine by Competitive Protein Binding (Displacement)Assay of Serum Thyroxine III. Semiautomated Determination of Thyroxine (T4) IodineVII. Special Techniques Paper Chromatography of Urine in the Detection of Metabolic Diseases Methods for Assuring Quality Data from Continuous-Flow Analyzers Application of Gel-Diffusion Techniques in the Clinical LaboratoryAuthor IndexSubject Index