Thrombosis and Bleeding Disorders

Thrombosis and Bleeding Disorders compiles the laboratory and research aspects of thrombosis and hemorrhagic disorders in humans. This book presents reviews of the underlying theory, physiology, and biochemistry of hemostasis and thrombosis, including the enzymology of blood coagulation and fibrinolysis. This compilation is divided into three levels of specific purposes. First is to provide the most reliable and widely accepted laboratory assays of undisputed diagnostic clinical value, which provides newcomers in the field and experienced workers in the coagulation laboratory with a reference manual to everyday work in a clinically-oriented environment. Second is to review and sketch in outline the theoretical sections focusing on mechanisms. Finally, this text aims to include a systematic review of the most successful purification techniques for individual coagulation factors and moieties of the fibrinolytic enzyme system. This publication is beneficial to medical students and clinicians concerned with human blood coagulation.

PrefaceChapter I Physiology and Biochemistry of Blood Coagulation Introduction Historical Aspects of Blood Coagulation Nomenclature Physicochemical Properties of Coagulation Constituents Activation of Prothrombin Conclusions and SummaryChapter II Equipment and General Requirements of the Coagulation Laboratory Introduction Equipment Collection of Blood Cleaning and Preparation of Glassware Buffer Solutions Anticoagulants Ion Exchange ResinsChapter III Clotting Time Techniques Introduction Whole Blood Coagulation Time Recalcification Time Heparin Sensitized Clotting Time Introduction Description of Methods General Discussion Thrombelastography Introduction Basic Description of Apparatus and Principle Techniques of Measurement Evaluation of the Thrombelastogram Abnormal Thrombelastographic Partial Thromboplastin Time Techniques Introduction Reagents Steps in Procedure Interpretation of Results Modifications Precautions Prothrombin Consumpdon Tests Introduction Measurement of Prothrombin Consumption Thrombin and Thromboplastin Generation Techniques Introduction Thrombin Generation Test Thromboplastin Generation Test Thromboplastin "Screening" Test (Thrombin- Thromboplastin Generation Test) Thromboplastin Activation Test One-Stage Prothrombin Time Techniques Introduction Practical Applications The Unmodified One-Stage Test The "P and P" Method (Owren and Aas, 1951) The Thrombotest Method (Owren 1959)Chapter IV Purification of Prothrombin Introduction Methods of Purification) Discussion Purification of Prethrombin "Modified Zymogen" Introduction Methods of Purification Discussion Purification of Thrombin Introduction Methods of Purification Discussion Purification of AC-Globulin (Factor V) Introduction Method of Purification Discussion Purification of Factor VIII Introduction Discussion Purification of Factor VII and Factor IX Introduction Factor VII - Methods of Purification Factor IX-Methods of Purification Discussion (Autoprothrombin III-C) Purification of Factor X Introduction Methods of Preparation Discussion Purification of Hageman Factor (Factor XII ) Introduction Methods of Purification Purification of Plasma Thromboplastin Antecedent (PTA, Factor XI) Introduction Methods of Purification Discussion Purification of Tissue Thromboplastin Introduction Methods of Preparation Purification of Platelet Factor 3 Introduction Method of Purification (Alkjaersig et al 1955) DiscussionChapter V Assay for Prothrombin Introduction Original Two-Stage Procedure Other Two-Stage Assays Role of Autoprothrombin C in the Assays Thrombin Clotting Assays Introduction Methods Assays of Factor V Introduction Methods of Assay Assays for Antihemophilic Factor (Factor VIII) Partial Thromboplastin Method (Langdell et al.